Covalent Linkage Between Ribonucleic Acid Primer and Deoxyribonucleic Acid Product of the Avian Myeloblastosis Virus Deoxyribonucleic Acid Polymerase
نویسندگان
چکیده
منابع مشابه
Covalent linkage between ribonucleic Acid primer and deoxyribonucleic Acid product of the avian myeloblastosis virus deoxyribonucleic Acid polymerase.
Initiation of deoxyribonucleic acid (DNA) synthesis by the avian myeloblastosis virus DNA polymerase was previously suggested to involve a ribonucleic acid (RNA) primer, the initial product being a DNA molecule joined by a phosphodiester bond to the RNA primer. The existence and nature of such an RNA-DNA joint was investigated by assaying for transfer of a (32)P atom from an alpha-(32)P-deoxyri...
متن کاملDeoxyribonucleic acid-dependent ribonucleic acid polymerase of Caulobacter crescentus.
Deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) was purified from the dimorphic bacterium Caulobacter crescentus at three stages in development. Enzyme from pure populations of stalked cells, as well as populations enriched in swarmer and predivisional cells, appeared identical in subunit structure and template requirements. The molecular weights of the enzyme sub...
متن کاملThe infidelity of avian myeloblastosis virus deoxyribonucleic acid polymerase in polynucleotide replication.
We have studied the accuracy of DNA synthesis in vitro by purified avian myeloblastosis virus (AMV) DNA polymerase (“reverse transcriptase”) using synthetic polynucleotide templates. The enzyme catalyzes the incorporation of an exceptionally large number of incorrectly paired bases when copying ribopolynucleotide and deoxyribopolynucleotide templates. The frequency of error is approximately 1 i...
متن کاملAssociation of an endoribonuclease with the avian myeloblastosis virus deoxyribonucleic acid polymerase.
A ribonuclease degrading only RNA complexed to DNA is found associated with the avian myeloblastosis virus DNA polymerase. A convenient and sensitive assay for the enzyme is degradation of [3H]poly(A) complexed to poly(dT). Using this assay, nuclease and DNA polymerase activities are inseparable by DEAE-Sephadex or phosphocellulose ion exchange chromatography or by glycerol gradient centrifugat...
متن کاملA primer ribonucleic acid for initiation of in vitro Rous sarcarcoma virus deoxyribonucleic acid synthesis.
The nucleotide sequence of an RNA primer molecule for initiation of Rous sarcoma virus DNA synthesis in vitro has been determined. The sequence can be drawn in a cloverleaf structure typical of tRNAs with an anticodon for tryptophan. Aminoacylation of the molecule confirms that it is tRNA-Trp. The same sequence and aminoacylation results are obtained regardless of whether the RNA is isolated fr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1972
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.10.4.622-627.1972